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  1. Zong G, Toonstra C, Yang Q, Zhang R, Wang L-X. Chemoenzymatic Synthesis and Antibody Binding of HIV-1 V1/V2 Glycopeptide-Bacteriophage Qβ Conjugates as a Vaccine Candidate. Int J Mol Sci. 2021, 22, 12538.

  2. Zhang X, Ou C, Liu H, Prabhu SK, Li C, Yang Q, Wang LX. General and Robust Chemoenzymatic Method for Glycan-Mediated Site-Specific Labeling and Conjugation of Antibodies: Facile Synthesis of Homogeneous Antibody-Drug Conjugates. ACS Chem Biol. 2021, doi: 10.1021/acschembio.1c00597. 

  3. Ou C, Li C, Zhang R, Yang Q, Zong G, Dai Y, Francis RL, Bournazos S, Ravetch JV, Wang LX. One-Pot Conversion of Free Sialoglycans to Functionalized Glycan Oxazolines and Efficient Synthesis of Homogeneous Antibody-Drug Conjugates through Site-Specific Chemoenzymatic Glycan Remodeling. Bioconjug Chem. 2021, 32, 1888-1897.

  4. Zhang X, Liu H, Meena N, Li C, Zong G, Raben N, Puertollano R, Wang LX. Chemoenzymatic glycan-selective remodeling of a therapeutic lysosomal enzyme with high-affinity M6P-glycan ligands. Enzyme substrate specificity is the name of the game. Chem Sci. 2021, 12, 12451-12462.

  5. Trastoy B, Du JJ, Li C, García-Alija M, Klontz EH, Roberts BR, Donahue TC, Wang LX, Sundberg EJ, Guerin ME. GH18 endo-β-N-acetylglucosaminidases use distinct mechanisms to process hybrid-type N-linked glycans. J Biol Chem. 2021, 297, 101011.

  6. Hargett AA, Marcella AM, Yu H, Li C, Orwenyo J, Battistel MD, Wang LX, Freedberg DI. Glycosylation States on Intact Proteins Determined by NMR Spectroscopy. Molecules. 2021, 26, 4308.

  7. Zong G, Li C, Prabhu SK, Zhang R, Zhang X, Wang LX. A facile chemoenzymatic synthesis of SARS-CoV-2 glycopeptides for probing glycosylation functions. Chem Commun. 2021, 57, 6804-6807.

  8. Prabhu SK, Li C, Zong G, Zhang R, Wang LX. Comparative studies on the substrate specificity and defucosylation activity of three α-l-fucosidases using synthetic fucosylated glycopeptides and glycoproteins as substrates. Bioorg Med Chem. 2021, 42, 116243.

  9. Li C, Chong G, Zong G, Knorr DA, Bournazos S, Aytenfisu AH, Henry GK, Ravetch JV, MacKerell AD Jr, Wang LX. Site-Selective Chemoenzymatic Modification on the Core Fucose of an Antibody Enhances Its Fcγ Receptor Affinity and ADCC Activity. J Am Chem Soc. 2021, 143, 7828-7838. 

  10. Sui Y, Li J, Zhang R, Prabhu SK, Andersen H, Venzon D, Cook A, Brown R, Teow E, Velasco J, Greenhouse J, Putman-Taylor T, Campbell TA, Pessaint L, Moore IN, Lagenaur L, Talton J, Breed MW, Kramer J, Bock KW, Minai M, Nagata BM, Lewis MG, Wang LX, Berzofsky JA. Protection against SARS-CoV-2 infection by a mucosal vaccine in rhesus macaques. JCI Insight. 2021, 6, e148494.

  11. Motabar D, Li J, Wang S, Tsao CY, Tong X, Wang LX, Payne GF, Bentley WE. Simple, rapidly electroassembled thiolated PEG-based sensor interfaces enable rapid interrogation of antibody titer and glycosylation. Biotechnol Bioeng. 2021, 118, 2744-2758.

  12. Li J, Wang SP, Zong G, Kim E, Tsao CY, VanArsdale E, Wang LX, Bentley WE, Payne GF. Interactive Materials for Bidirectional Redox-Based Communication. Adv Mater. 2021, 33, e2007758.

  13. Zhang R, Yang Q, Boruah BM, Zong G, Li C, Chapla D, Yang JY, Moremen KW, Wang LX. Appropriate aglycone modification significantly expands the glycan substrate acceptability of α1,6-fucosyltransferase (FUT8). Biochem J. 2021, 478, 1571-1583.

  14. ​Prabhu SK, Yang Q, Tong X, Wang LX. Exploring a combined Escherichia coli-based glycosylation and in vitro transglycosylation approach for expression of glycosylated interferon alpha. Bioorg Med Chem. 2021, 33, 116037.

  15. Yang Y, Li X, Ma Z, Wang C, Yang Q, Byrne-Steele M, Hong R, Min Q, Zhou G, Cheng Y, Qin G, Youngyunpipatkul JV, Wing JB, Sakaguchi S, Toonstra C, Wang LX, Vilches-Moure JG, Wang D, Snyder MP, Wang JY, Han J, Herzenberg LA. CTLA-4 expression by B-1a B cells is essential for immune tolerance. Nat Commun. 2021, 12, 525.



  1. Li J, Gray KM, Kim E, Conrad C, Tsao CY, Wang S, Zong G, Scarcelli G, Stroka KM, Wang LX, Bentley WE, Payne GF. Mediated Electrochemistry to Mimic Biology's Oxidative Assembly of Functional Matrices. Adv Funct Mater. 2020, 30, 2001776.

  2. Borghi S, Bournazos S, Thulin NK, Li C, Gajewski A, Sherwood RW, Zhang S, Harris E, Jagannathan P, Wang LX, Ravetch JV, Wang TT. FcRn, but not FcγRs, drives maternal-fetal transplacental transport of human IgG antibodies. Proc Natl Acad Sci USA. 2020, 117,12943-12951.

  3. Vasta GR, Feng C, Tasumi S, Abernathy K, Bianchet MA, Wilson IBH, Paschinger K, Wang LX, Iqbal M, Ghosh A, Amin MN, Smith B, Brown S, Vista A. Biochemical Characterization of Oyster and Clam Galectins: Selective Recognition of Carbohydrate Ligands on Host Hemocytes and Perkinsus Parasites. Front Chem. 2020, 8, 98.

  4. Lin L, Kightlinger W, Prabhu SK, Hockenberry AJ, Li C, Wang LX, Jewett MC, Mrksich M. Sequential Glycosylation of Proteins with Substrate-Specific N-Glycosyltransferases. ACS Cent Sci. 2020, 6, 144-154.

  5. Trastoy B, Du JJ, Klontz EH, Li C, Cifuente JO, Wang LX, Sundberg EJ, Guerin ME. Structural basis of mammalian high-mannose N-glycan processing by human gut Bacteroides. Nat Commun. 2020, 11, 899.

  6. Zong G, Li C, Wang LX. Chemoenzymatic Synthesis of HIV-1 Glycopeptide Antigens. Peptide Synthesis. Methods in Molecular Biology 2020, 2103, 249-262.



  1. Wang LX, Tong X, Li C, Giddens JP, Li T. Glycoengineering of Antibodies for Modulating Functions. Annu Rev Biochem. 2019, 88, 433-459.

  2. Klontz EH, Trastoy B, Deredge D, Fields JK, Li C, Orwenyo J, Marina A, Beadenkopf R, Günther S, Flores J, Wintrode PL, Wang LX, Guerin ME, Sundberg EJ. Molecular Basis of Broad Spectrum N-Glycan Specificity and Processing of Therapeutic IgG Monoclonal Antibodies by Endoglycosidase S2. ACS Cent Sci. 2019, 5, 524-538.


  1. Giddens JP, Lomino JV, DiLillo DJ, Ravetch JV, Wang LX. Site-selective chemoenzymatic glycoengineering of Fab and Fc glycans of a therapeutic antibody. Proc Natl Acad Sci U S A. 2018, doi: 10.1073/pnas.1812833115.

  2. Cai H, Zhang R, Orwenyo J, Giddens JP, Yang Q, LaBranche CC, Montefiori D, Wang LX. Synthetic HIV V3 Glycopeptide Immunogen Carrying a N334 N-Glycan Induces Glycan-dependent Antibodies with Promiscuous Site Recognition. J Med Chem. 2018, doi: 10.1021/acs.jmedchem.8b01290.

  3. Chemoenzymatic Defucosylation of Therapeutic Antibodies for Enhanced Effector Functions Using Bacterial α-Fucosidases. Li C, Li T, Wang LX. Methods Mol Biol. 2018, 1827, 367-380.

  4. Chemoenzymatic Methods for the Synthesis of Glycoproteins. Li C, Wang LX. Chem Rev. 2018, 118, 8359-8413.

  5. Generation and Comparative Kinetic Analysis of New Glycosynthase Mutants From Streptococcus pyogenes Endoglycosidases For Antibody Glycoengineering. Tong X, Li T, Li C, Wang LX. Biochemistry 2018, 57, 5239-5246.

  6. The Odd "RB" Phage-Identification of Arabinosylation as a New Epigenetic Modification of DNA in T4-Like Phage RB69. Thomas JA, Orwenyo J, Wang LX, Black LW. Viruses. 2018, 10, E313.

  7. Multivalent Antigen Presentation Enhances the Immunogenicity of a Synthetic Three-Component HIV-1 V3 Glycopeptide Vaccine. Cai H, Zhang R, Orwenyo J, Giddens J, Yang Q, LaBranche CC, Montefiori DC, Wang LX. ACS Cent Sci. 2018, 4, 582-589.

  8. Structural basis for the recognition of complex-type N-glycans by Endoglycosidase S. Trastoy B, Klontz E, Orwenyo J, Marina A, Wang LX, Sundberg EJ, Guerin ME. Nat Commun. 2018, 9, 1874.

  9. Top-Down Chemoenzymatic Approach to Synthesizing Diverse High-Mannose N-Glycans and Related Neoglycoproteins for Carbohydrate Microarray Analysis. Toonstra C, Wu L, Li C, Wang D, Wang LX. Bioconjug Chem. 2018 , 29, 1911-1921.

  10. Li T, Li C, Quan DN, Bentley WE, Wang LX. Site-specific immobilization of endoglycosidases for streamlined chemoenzymatic glycan remodeling of antibodies. Carbohydr Res. 2018, 458-459, 77-84.

  11. Bennett LD, Yang Q, Berquist BR, Giddens JP, Ren Z, Kommineni V, Murray RP, White EL, Holtz BR, Wang LX, Marcel S. Implementation of Glycan Remodeling to Plant-Made Therapeutic Antibodies. Int J Mol Sci. 2018, E421.


  1. Li C, Zhu S, Tong X, Ou C, Wang LX. A facile synthesis of a complex type Nglycan thiazoline as an effective inhibitor against the antibody-deactivating endo-β-Nacetylglucosaminidases. J Carbohyd Chem. 2017, 36, 336-346.

  2. Cai H, Orwenyo J, Giddens JP, Yang Q, Zhang R, LaBranche CC, Montefiori DC, Wang LX. Synthetic Three-Component HIV-1 V3 Glycopeptide Immunogens Induce Glycan-Dependent Antibody Responses. Cell Chem Biol. 2017, 24, 1513-1522.e4.

  3. Li C, Zhu S, Ma C, Wang LX. Designer α1,6-Fucosidase Mutants Enable Direct Core Fucosylation of Intact N-Glycopeptides and N-Glycoproteins. J Am Chem Soc. 2017, 139, 15074-15087.

  4. Yang Q, Wang LX. Chemoenzymatic Glycan Remodeling of Natural and Recombinant Glycoproteins. Methods Enzymol. 2017, 597, 265-281.

  5. Tong X, Li T, Orwenyo J, Toonstra C, Wang LX. One-pot enzymatic glycan remodeling of a therapeutic monoclonal antibody by endoglycosidase S (Endo-S) from Streptococcus pyogenes. Bioorg Med Chem. 2017 doi: 10.1016/j.bmc.2017.07.053.

  6. Tang F, Wang LX, Huang W. Chemoenzymatic synthesis of glycoengineered IgG antibodies and glycosite-specific antibody-drug conjugates. Nat Protoc. 2017, 12, 1702-1721.

  7. Yang Q, Zhang R, Cai H, Wang LX. Revisiting the substrate specificity of mammalian α1,6-fucosyltransferase (FUT8) reveals that it catalyzes core fucosylation of N-glycans lacking α1,3-arm GlcNAc. J Biol Chem. 2017, 292, 14796-14803.

  8. Yang M, Huang J, Simon R, Wang LX, MacKerell AD Jr. Conformational Heterogeneity of the HIV Envelope Glycan Shield. Sci Rep. 2017, 30, 4435.

  9. Feng C, Li J, Snyder G, Huang W, Goldblum SE, Chen WH, Wang LX, McClane BA, Cross AS. Antibody against Microbial Neuraminidases Recognizes Human Sialidase 3 (NEU3): the Neuraminidase/Sialidase Superfamily Revisited. MBio. 2017, 8, e00078-17.

  10. Cai H, Orwenyo J, Guenaga J, Giddens J, Toonstra C, Wyatt RT, Wang LX. Synthetic multivalent V3 glycopeptides display enhanced recognition by glycan-dependent HIV-1 broadly neutralizing antibodies. Chem Commun (Camb). 2017, 53, 5453-5456.

  11. Yang Q, An Y, Zhu S, Zhang R, Loke CM, Cipollo JF, Wang LX. Glycan Remodeling of Human Erythropoietin (EPO) Through Combined Mammalian Cell Engineering and Chemoenzymatic Transglycosylation. ACS Chem Biol. 2017, 12, 1665-1673.

  12. Orwenyo J, Cai H, Giddens J, Amin MN, Toonstra C, Wang LX. Systematic Synthesis and Binding Study of HIV V3 Glycopeptides Reveal the Fine Epitopes of Several Broadly Neutralizing Antibodies. ACS Chem. Biol. 2017, 12, 1566-1575.

  13. Li T, DiLillo DJ, Bournazos S, Giddens JP, Ravetch JV, Wang LX. Modulating IgG effector function by Fc glycan engineering. Proc. Natl. Acad. Sci. U S A. 2017, 114, 3485-3490.

  14. Li C, Wang LX. Chemical Biology of Protein N-Glycosylation. Chemical Biology of Glycoproteins. Royal Society of Chemistry press. 2017, pp 20-47.

  15. Freund NT, Wang H, Scharf L, Nogueira L, Horwitz JA, Bar-On Y, Golijanin J, Sievers SA, Sok D, Cai H, Cesar Lorenzi JC, Halper-Stromberg A, Toth I, Piechocka-Trocha A, Gristick HB, van Gils MJ, Sanders RW, Wang LX, Seaman MS, Burton DR, Gazumyan A, Walker BD, West AP Jr, Bjorkman PJ, Nussenzweig MC. Coexistence of potent HIV-1 broadly neutralizing antibodies and antibody-sensitive viruses in a viremic controller. Sci Transl Med 2017, 9, eaal2144.


  1. Li C, Wang LX. Endoglycosidases for the Synthesis of Polysaccharides and Glycoconjugates. Adv Carbohydr Chem Biochem 2016, 23, 73-116.

  2. Hsiao PY, Kalin JH, Sun IH, Amin MN, Lo YC, Chiang MJ, Giddens J, Sysa-Shah P, Gabrielson K, Wang LX, Powell JD, Cole PA. An Fc-Small Molecule Conjugate for Targeted Inhibition of the Adenosine 2A Receptor. Chembiochem 2016, 20, 1951-1960.

  3. Yamaguchi T, Amin MN, Toonstra C, Wang LX. Chemoenzymatic Synthesis and Receptor Binding of Mannose-6-Phosphate (M6P)-Containing Glycoprotein Ligands Reveal Unusual Structural Requirements for M6P Receptor Recognition. J Am Chem Soc 2016, 138, 12472-12485.

  4. Li T, Tong X, Yang Q, Giddens JP, Wang LX. Glycosynthase Mutants of Endoglycosidase S2 Show Potent Transglycosylation Activity and Remarkably Relaxed Substrate Specificity for Antibody Glycosylation Remodeling. J Biol Chem 2016, 291, 16508-16518.

  5. Toonstra C, Amin MN, Wang LX. Site-Selective Chemoenzymatic Glycosylation of an HIV-1 Polypeptide Antigen with Two Distinct N-Glycans via an Orthogonal Protecting Group Strategy. J Org Chem 2016, 81, 6176-6185.

  6. Yang Q, Wang LX. Mammalian α-1,6-Fucosyltransferase (FUT8) Is the Sole Enzyme Responsible for the N-Acetylglucosaminyltransferase I-independent Core Fucosylation of High-mannose N-Glycans. J Biol Chem 2016, 291, 11064-11071.

  7. Giddens JP, Lomino JV, Amin MN, Wang LX. Endo-F3 Glycosynthase Mutants Enable Chemoenzymatic Synthesis of Core-fucosylated Triantennary Complex Type Glycopeptides and Glycoproteins. J Biol Chem 2016, 291, 9356-9370.

  8. Liu R, Giddens J, McClung CM, Magnelli PE, Wang LX, Guthrie EP. Evaluation of a glycoengineered monoclonal antibody via LC-MS analysis in combination with multiple enzymatic digestion. MAbs 2016, 29, 340-346.



  1. Quast I, Keller CW, Maurer MA, Giddens JP, Tackenberg B, Wang LX, Münz C, Nimmerjahn F, Dalakas MC, Lünemann JD. “ialylation of IgG Fc domain impairs complement-dependent cytotoxicity. J Clin Invest 2015, 125, 4160-4170.

  2. Feng C, Ghosh A, Amin MN, Bachvaroff TR, Tasumi S, Pasek M, Banerjee A, Shridhar S, Wang LX, Bianchet MA, Vasta GR. Galectin CvGal2 from the Eastern Oyster (Crassostrea virginica) Displays Unique Specificity for ABH Blood Group Oligosaccharides and Differentially Recognizes Sympatric Perkinsus Species. Biochemistry 2015, 54, 4711-4730.

  3. Giddens JP, Wang LX. Chemoenzymatic Glyco-engineering of Monoclonal Antibodies. Methods Mol Biol 2015, 1321, 375-387.

  4. Nita-Lazar M, Banerjee A, Feng C, Amin MN, Frieman MB, Chen WH, Cross AS, Wang LX, Vasta GR. Desialylation of airway epithelial cells during influenza virus infection enhances pneumococcal adhesion via galectin binding. Mol Immunol 2015, 65, 1-16.

  5. Wang D, Tang J, Tang J, Wang LX. Targeting N-glycan cryptic sugar moieties for broad-spectrum virus neutralization: progress in identifying conserved molecular targets in viruses of distinct phylogenetic origins. Molecules 2015, 20, 4610-4622.



  1. Ahmed AA, Giddens J, Pincetic A, Lomino JV, Ravetch JV, Wang LX, Bjorkman PJ. Structural characterization of anti-inflammatory immunoglobulin G Fc proteins. J Mol Biol 2014, 426, 3166-3179.

  2. Neundlinger I, Puntheeranurak T, Wildling L, Rankl C, Wang LX, Gruber HJ, Kinne RK, Hinterdorfer P. Forces and dynamics of glucose and inhibitor binding to sodium glucose co-transporter SGLT1 studied by single molecule force spectroscopy. J Biol Chem 2014, 289, 21673-21683.

  3. Wang D, Bhat R, Sobel RA, Huang W, Wang LX, Olsson T, Steinman L. Uncovering cryptic glycan markers in multiple sclerosis (MS) and experimental autoimmune encephalomyelitis (EAE). Drug Dev Res 2014, 75, 172-188.

  4. Trastoy B, Lomino JV, Pierce BG, Carter LG, Günther S, Giddens JP, Snyder GA, Weiss TM, Weng Z, Wang LX, Sundberg EJ. Crystal structure of Streptococcus pyogenes EndoS, an immunomodulatory endoglycosidase specific for human IgG antibodies. Proc Natl Acad Sci U S A 2014, 111, 6714-6719.

  5. Smith EL, Giddens JP, Iavarone AT, Godula K, Wang LX, Bertozzi CR. Chemoenzymatic Fc glycosylation via engineered aldehyde tags. Bioconjug Chem 2014, 25, 788-795.

  6. Lillehoj EP, Hyun SW, Feng C, Zhang L, Liu A, Guang W, Nguyen C, Sun W, Luzina IG, Webb TJ, Atamas SP, Passaniti A, Twaddell WS, Puché AC, Wang LX, Cross AS, Goldblum SE. Human airway epithelia express catalytically active NEU3 sialidase. Am J Physiol Lung Cell Mol Physiol. 2014, 306, L876-86.

  7. Heredia A, Davis C, Amin MN, Le NM, Wainberg MA, Oliveira M, Deeks SG, Wang LX, Redfield RR. Targeting host nucleotide biosynthesis with resveratrol inhibits emtricitabine-resistant HIV-1. AIDS 2014, 28, 317-323.

  8. Wang LX, Amin MN. Chemical and chemoenzymatic synthesis of glycoproteins for deciphering functions, Chem Biol. 2014, 21, 51-66.

  9. Chiang MJ, Holbert MA, Kalin JH, Ahn YH, Giddens J, Amin MN, Taylor MS, Collins SL, Chan-Li Y, Waickman A, Hsiao PY, Bolduc D, Leahy DJ, Horton MR, Wang LX, Powell JD, Cole PA. An Fc domain protein-small molecule conjugate as an enhanced immunomodulator. J. Am. Chem. Soc. 2014, 136, 3370-3373.



  1. Wang LX. Synthetic carbohydrate antigens for HIV vaccine design, Curr Opin Chem Biol. 2013, 17, 997-1005.

  2. Wang LX, Davis BG. Realizing the promise of chemical glycobiology, Chem Sci., 2013, 4, 3381-3394.

  3. Trastoy B, Lomino JV, Wang LX, Sundberg EJ. Liquid-liquid diffusion crystallization improves the X-ray diffraction of EndoS, an endo-β-N-acetylglucosaminidase from Streptococcus pyogenes with activity on human IgG. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2013, 69, 1405-10.

  4. Amin MN, McLellan JS, Huang W, Orwenyo J, Burton DR, Koff WC, Kwong PD, Wang LX. Synthetic glycopeptides reveal the glycan specificity of HIV-neutralizing antibodies. Nature Chem. Biol. 2013, 9, 521-526.

  5. Pancera M, Shahzad-Ul-Hussan S, Doria-Rose NA, McLellan JS, Bailer RT, Dai K, Loesgen S, Louder MK, Staupe RP, Yang Y, Zhang B, Parks R, Eudailey J, Lloyd KE, Blinn J, Alam SM, Haynes BF, Amin MN, Wang LX, Burton DR, Koff WC, Nabel GJ, Mascola JR, Bewley CA, Kwong PD. Structural basis for diverse N-glycan recognition and enhanced HIV-1 neutralization by V1/V2-directed antibodies. Nature Struct. Mol. Biol. 2013, 20, 804-813.

  6. Orwenyo J, Huang W, Wang LX. Chemoenzymatic synthesis and lectin recognition of a selectively fluorinated glycoprotein. Bioorg. Med. Chem. 2013, 21, 4768-77.

  7. Lomino JV, Naegeli A, Orwenyo J, Amin MN, Aebi M, Wang LX. A two-step enzymatic glycosylation of polypeptides with complex N-glycans. Bioorg. Med. Chem. 2013, 21, 2262-70.

  8. Wang H, Huang W, Orwenyo J, Banerjee A, Vasta GR, Wang LX. Design and synthesis of glycoprotein-based multivalent glyco-ligands for influenza hemagglutinin and human galectin-3. Bioorg. Med. Chem. 2013, 21, 2037-2044.

  9. Feng C, Ghosh A, Amin MN, Giomarelli B, Shridhar S, Banerjee A, Fernández-Robledo JA, Bianchet MA, Wang LX, Wilson IB, Vasta GR. The galectin CvGal1 from the eastern oyster (Crassostrea virginica) binds to blood group A oligosaccharides on the hemocyte surface. J. Biol. Chem. 2013, 288, 24394-24409.

  10. Wang D, Dafik L, Nolley R, Huang W, Wolfinger RD, Wang LX, Peehl DM. Anti-Oligomannose Antibodies as Potential Serum Biomarkers of Aggressive Prostate Cancer. Drug Dev Res. 2013, 74, 65-80.


  1. Huang W, Giddens J, Fan SQ, Toonstra C, Wang LX. Chemoenzymatic glycoengineering of intact IgG antibodies for gain of functions. J. Am. Chem. Soc, 2012, 134, 12308−12318.

  2. Cross AS, Hyun SW, Miranda-Ribera A, Feng C, Liu A, Nguyen C, Zhang L, Luzina IG, Atamas SP, Twaddell WS, Guang W, Lillehoj EP, Puché AC, Huang W, Wang LX, Passaniti A, Goldblum SE. NEU1 and NEU3 sialidase activity expressed in human lung microvascular endothelia. NEU1 restrains endothelial cell migration whereas NEU3 does not. J. Biol. Chem. 2012, 287, 15966-15980.

  3. Fan SQ, Huang W, Wang LX. Remarkable transglycosylation activity of glycosynthase mutants of Endo-D, an endo-β-N-acetylglucosaminidase from Streptococcus pneumoniae. J. Biol. Chem., 2012, 287, 11272–11281.

  4. Lillehoj EP, Hyun SW, Feng C, Zhang L, Liu A, Guang W, Nguyen C, Luzina IG, Atamas SP, Passaniti A, Twaddell WS, Puché AC, Wang LX, Cross AS, Goldblum SE. NEU1 sialidase expressed in human airway epithelia regulates epidermal growth factor receptor (EGFR) and MUC1 protein signaling. J. Biol. Chem. 2012, 287, 8214-8231.

  5. Wang LX, Lomino JV. Emerging technologies for making glycan-defined glycoproteins. ACS Chem Biol. 2012, 7, 110-22.



  1. McLellan JS, Pancera M, Carrico C, Gorman J, Julien JP, Khayat R, Louder R, Pejchal R, Sastry M, Dai K, O'Dell S, Patel N, Shahzad-ul-Hussan S, Yang Y, Zhang B, Zhou T, Zhu J, Boyington JC, Chuang GY, Diwanji D, Georgiev I, Kwon YD, Lee D, Louder MK, Moquin S, Schmidt SD, Yang ZY, Bonsignori M, Crump JA, Kapiga SH, Sam NE, Haynes BF, Burton DR, Koff WC, Walker LM, Phogat S, Wyatt R, Orwenyo J, Wang LX, Arthos J, Bewley CA, Mascola JR, Nabel GJ, Schief WR, Ward AB, Wilson IA, Kwong PD. Structure of HIV-1 gp120 V1/V2 domain with broadly neutralizing antibody PG9. Nature 2011, 480, 336-343.

  2. Zou G, Ochiai H, Huang W, Yang Q, Li C, Wang LX. Chemoenzymatic synthesis and Fcg receptor binding of homogeneous glycoforms of antibody Fc domain. Presence of a bisecting sugar moiety enhances the affinity of Fc to FcgIIIa receptor. J. Am. Chem. Soc. 2011, 133, 18975-18991.

  3. Amin MN, Huang W, Mizanur RM, Wang LX. Convergent synthesis of homogeneous Glc1Man9GlcNAc2-protein and derivatives as ligands of molecular chaperones in protein quality control. J. Am. Chem. Soc., 2011, 133, 14404–14417.

  4. Li Y, Qiao H, Xing C, Zhang J, Wang LX, Wang H, Zhang B, Tang J. Molecular recognition force spectroscopy of a specific lectin-carbohydrate interaction at single-molecule level. J Struct Biol. 2011, 176, 46-51.

  5. Zhou D, Levery SB, Hsu FF, Wang PG, Teneberg S, Almeida IC, Li Y, Xu H, Wang LX, Xia C, Ibrahim NK, Michael K. Front Biosci (Schol Ed). 2011, 3, 1520-32.

  6. Huang W, Li J, Wang LX. Unusual transglycosylation activity of Flavobacterium meningosepticum endoglycosidases enables convergent chemoenzymatic synthesis of core fucosylated complex N-glycopeptides. ChemBioChem 2011, 12, 932-941.

  7. Wang LX. The Amazing Transglycosylation Activity of Endo-β-N-acetylglucosaminidases. Trends Glycosci. Glycotechnol. 2011, 23, 33-52.



  1. Huang W, Zhang X, Ju T, Cummings RD, Wang LX. Expeditious chemoenzymatic synthesis of CD52 glycopeptide antigens. Org. Biomol. Chem. 2010, 8, 5224-5233.

  2. Stamatos NM, Carubelli I, van de Vlekkert D, Bonten EJ, Papini N, Feng C, Venerando B, d'Azzo A, Cross AS, Wang LX, Gomatos PJ. J Leukoc Biol. 2010, 88, 1227-39.

  3. Brooks CL, Schietinger A, Borisova SN, Kufer P, Okon M, Hirama T, Mackenzie CR, Wang LX, Schreiber H, Evans SV. Antibody recognition of a novel tumor-specific glycopeptide antigen. Proc. Natl. Acad. Sci. USA 2010, 107, 10056-10061.

  4. Huang W, Yang Q, Umekawa M, Yamamoto K, Wang LX. Arthrobacter endo-beta-N-acetylglucosaminidase shows transglycosylation activity on complex type N-glycan oxazolines. One-pot conversion of ribonuclease B to sialylated ribonuclease C. ChemBioChem 2010, 11, 1350-1355.

  5. Yang Q, Li C, Wei Y, Huang W, Wang LX. Expression, glycoform characterization, and antibody-binding of HIV-1 V3 glycopeptide domain fused with human IgG1-Fc. Bioconjugate Chem. 2010, 21, 875-883.

  6. Schwarz F, Huang W, Li C, Schulz BL, Lizak C, Palumbo A, Numao S, Neri D, Aebi M, Wang LX. A combined method for producing homogeneous glycoproteins with eukaryotic N-glycosylation. Nature Chem. Biol. 2010, 6, 264-266.

  7. Umekawa M, Higashiyama T, Koga Y, Tanaka T, Noguchi M, Kobayashi A, Shoda S, Huang W, Wang LX, Ashida H, Yamamoto K. Efficient transfer of sialo-oligosaccharide onto proteins by combined use of a glycosynthase-like mutant of Mucor hiemalis endoglycosidase and synthetic sialo-complex-type sugar oxazoline. Biochim Biophys Acta. 2010, 1800, 1203-9.

  8. Umekawa M, Li C, Higashiyama T, Huang W, Ashida H, Yamamoto K, Wang LX. Efficient glycosynthase mutant derived from Mucor hiemalis endo-beta-N-acetylglucosaminidase capable of transferring oligosaccharide from both sugar oxazoline and natural N-glycan. J Biol Chem. 2010, 285, 511-21.



  1. Huang W, Wang D, Yamada M, Wang LX. Chemoenzymatic synthesis and lectin array characterization of a class of N-glycan clusters. J. Am. Chem. Soc. 2009, 131, 17963-17971.

  2. Luallen RJ, Fu H, Agrawal-Gamse C, Mboudjeka I, Huang W, Lee FH, Wang LX, Doms RW, Geng Y. A yeast glycoprotein shows high-affinity binding to the broadly neutralizing human immunodeficiency virus antibody 2G12 and inhibits gp120 interactions with 2G12 and DC-SIGN. J Virol. 2009, 83, 4861-70.

  3. Yin J, Li L, Shaw N, Li Y, Song JK, Zhang W, Xia C, Zhang R, Joachimiak A, Zhang HC, Wang LX, Liu ZJ, Wang P. Structural basis and catalytic mechanism for the dual functional endo-beta-N-acetylglucosaminidase A. PLoS One 2009, 4, e4658.

  4. Huang W, Li C, Li B, Umekawa M, Yamamoto K, Zhang X, Wang LX. Novel glycosynthases enable a highly efficient chemo-enzymatic synthesis of N-glycoproteins carrying intact natural N-glycans. J. Am. Chem. Soc. 2009, 131, 2214-2223.

  5. Zhu XY, Holtz B, Wang Y, Wang LX, Orndorff PE, Guo A. Quantitative glycomics from fluidic glycan microarrays. J. Am. Chem. Soc. 2009, 131, 13646-13650.

  6. Wang LX. Expanding the repertoire of glycosynthases. Chem. Biol. 2009, 16, 1026-1027.

  7. Newsom-Davis TE, Wang D, Steinman L, Chen PF, Wang LX, Simon AK, Screaton GR. Enhanced immune recognition of cryptic glycan markers in human tumors. Cancer Res. 2009, 69, 2018-25.

  8. Wang LX, Huang W. Enzymatic transglycosylation for glycoconjugate synthesis. Curr. Opin. Chem. Biol, 2009, 13, 592–600.

  9. Huang W, Groothuys S, Heredia A, Kuijpers BH, Rutjes FP, van Delft FL, Wang LX. Enzymatic glycosylation of triazole-linked GlcNAc/Glc-peptide: Synthesis, stability, and anti-HIV activity of triazole-linked HIV-1 gp41 glycopeptide C34 analogues. ChemBioChem 2009, 10, 1234-1242.

  10. Ochiai H, Huang W, Wang LX. Endo-beta-N-acetylglucosaminidase-catalyzed polymerization of beta-Glcp-(1-->4)-GlcpNAc oxazoline: a revisit to enzymatic transglycosylation. Carbohydr Res. 2009, 344, 592-8.

  11. Chan SL, Voskens CJ, Lin W, Schindler DG, Azimzadeh A, Wang LX, Taylor RJ, Strome SE, Schulze DH. J Mol Recognit. 2009, 22, 242-9.



  1. Huang W, Ochiai H, Zhang X, Wang LX. Introducing N-glycans into natural products through a chemoenzymatic approach. Carbohydr. Res. 2008, 343, 2903-2913.

  2. Wei Y, Li C, Huang W, Li B, Strome S, Wang LX. Glyco-engineering of human IgG1-Fc through combined yeast expression and in vitro chemoenzymatic glycosylation. Biochemistry 2008, 47, 10294-10304.

  3. Ochiai H, Huang W, Wang LX. Expeditious chemoenzymatic synthesis of homogeneous N-glycoproteins carrying defined oligosaccharide ligands. J. Am. Chem. Soc. 2008, 130, 13790-13803.

  4. Li C, Huang W, Wang LX. Chemoenzymatic synthesis of N-linked neoglycoproteins through a chitinase-catalyzed transglycosylation. Bioorg. Med. Chem. 2008, 16, 8366-8372.

  5. Lin W, Voskens CJ, Zhang X, Schindler DG, Wood A, Burch E, Wei Y, Chen L, Tian G, Tamada K, Wang LX, Schulze DH, Mann D, Strome SE. Blood 2008, 112, 699-707.

  6. Li B, Takegawa K, Suzuki T, Yamamoto K, Wang LX. Synthesis and inhibitory activity of oligosaccharide thiazolines as a class of mechanism-based inhibitors for endo-beta-N-acetylglucosaminidases. Bioorg. Med. Chem. 2008, 16, 4670-4675.

  7. Wang LX. Chemoenzymatic synthesis of glycopeptides and glycoproteins through endoglycosidase-catalyzed transglycosylation. Carbohydr. Res. 2008, 343, 1509-1522.

  8. Umekawa M, Huang W, Li B, Fujita K, Ashida H, Wang LX, Yamamoto K. Mutants of Mucor hiemalis endo-beta-N-acetylglucosaminidase show enhanced transglycosylation and glycosynthase-like activities. J. Biol. Chem. 2008, 283, 4469-4479.

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